VIADENT Fluorescence

The only option for obtaining Counts, Motility, and Viability from a Single Motile Sperm Sample

• Recent Abstract
• Why Choose VIADENT
• How VIADENT Works
• Easy Sample Preparation
• The Analysis Process

Recent Abstract

Validation of an objective approach for simultaneous assessment of viability and motility of fresh and cooled equine spermatozoa.
M.T. Wessel, G.C. Althouse
Proceedings of the International Symposium on Equine Reproduction, Animal Reproduction Science 94 (2006) 21-22

"Our results suggest that the "Viadent" option of the IVOS system is capable of a rapid, accurate and objective evaluation of both viability and motility parameters using large numbers of spermatozoa. Application of this technique in the industry may prove useful in the clinical assessment of fertilizing potential of equine spermatozoa."
(Read Full Abstract)

Why Choose VIADENT

• Only VIADENT uses the same MOTILE sample for counts, motility, and viability. Using other viability options, two separate samples must be analyzed for motility, counts, and viability.
• VIADENT only uses one stain, and staining is complete within 2 minutes. Other methods require 2 to 3 separate stains, and much longer staining times.
• VIADENT is compatible with clear, milk-based, and egg-yolk extenders. However, use of phosphate-buffered solutions may interfere with other viability stains, such as SYBR 14.
• Low cost per test. Each VIADENT stain tube provides enough stain for 5 samples, and may be refrigerated after reconstitution for up to 3 months.
• Future applications under development.

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How it Works

The VIADENT stain only penetrates non-viable sperm heads. By comparing the cells visible under standard and fluorescent illumination, the analyzer easily determines the live cells versus the dead cells.

• The ‘visible’ Playback screen (see image below) shows all the sperm cells in a single field.
• The ‘fluorescent’ Playback screen shows only the dead cells. These non-viable cells are identified by a red square on both screens.
• Green tracks indicate motile sperm, and red dots indicate non-motile sperm (both live and dead).

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Easy Sample Preparation

Simply stain an aliquot of the sample with the pre-packaged VIADENT stain for 2 minutes, load into the disposable analysis chamber, and place on the IVOS or UltiMate stage. You are now ready to begin analysis!

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The Analysis Process

1) Select the analysis fields either automatically or manually.

2) Press Start Scan. Using standard visible illumination, the system analyzes count, concentration, motility, and velocity.

3) Select Playback to confirm capture.

4) Activate the VIADENT illumination, and press ADD SCAN. The analyzer will automatically scan the identical fields under fluorescent illumination to detect non-viable sperm.

5) The analysis results are immediately displayed, and show the number, concentration, and percent Viable cells, in addition to the Total, Motile, and Progressive values. You also get the quality assurance of overlaying Playback of both runs so you can “see” the results.

Note: VIADENT is available only on IVOS, TOX IVOS, and UltiMate systems with the IDENT fluorescent option installed.

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