XYClone is for non-clinical research use only in USA. hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations. Please Note: The XYClone™ is for use ONLY with ANIMAL and STEM CELL APPLICATIONS. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)
F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses.
William T Poueymirou, Wojtek Auerbach, David Frendewey, Joseph F Hickey, Jennifer M Escaravage, Lakeisha Esau, Anthony T Dore´, Sean Stevens, Niels C Adams, Melissa G Dominguez, Nicholas W Gale, George D Yancopoulos, Thomas M DeChiara& David M Valenzuela.
Nature Biotechnology, 2007. Jan. 25(1):91-99.
A useful approach for exploring gene function involves generating mutant mice from genetically modified embryonic stem (ES) cells. Recent advances in genetic engineering of ES cells have shifted the bottleneck in this process to the generation of mice. Conventional injections of ES cells into blastocyst hosts produce F0 generation chimeras that are only partially derived from ES cells, requiring additional breeding to obtain mutant mice that can be phenotyped. The tetraploid complementation approach directly yields mice that are almost entirely derived from ES cells, but it is inefficient, works only with certain hybrid ES cell lines and suffers from nonspecific lethality and abnormalities, complicating phenotypic analyses. Here we show that laser assisted injection of either inbred or hybrid ES cells into eight cell–stage embryos efficiently yields F0 generation mice that are fully ES cell–derived and healthy, exhibit 100% germline transmission and allow immediate phenotypic analysis, greatly accelerating gene function assignment.
Chimera mouse and VelociMouse (Photo courtesy Ian Rosewell, Cancer Research UK)
After receiving a demonstration of the XYClone at their laboratory, they learned that the laser could also replace the sharp micropipettes they used for blastocyst injection. When comparing methods, they find that the “insertion of cells into the blastocysts cavity is easier with the XYClone and causes less damage to the blastocysts” than with their old micropipette method. Read the full testimonial.
XYClone for Laser-assisted Injections 