Laser-assisted Hatching

The advent of the laser has allowed the development of precision techniques to manipulate embryos for enhanced fertility. Laser-assisted hatching can turn around a history of failure for embryos to implant themselves in the uterine wall.

Assisted hatching is used to help the embryo hatch from its protective outer shell, the zona pellucida, and promote implantation in the uterine wall after embryo transfer. Laser-assisted hatching (LAH) with the LYKOS® or ZILOS-tk® uses a highly focused infrared laser beam to remove the zona pellucida in very precise increments. Prior to the clinical availability of the lasers, only mechanical or chemical methods could be used for assisted hatching of human embryos in clinical settings. Laser-assisted hatching requires less handling of the embryo than these other assisted hatching methods. Also, laser-assisted hatching is faster than the other methods and, therefore, the embryo spends less time outside the incubator.

Clinical Laser-assisted Hatching

Indications for LAH


Women over 37 years old

Hormonal Status

Women with an elevated baseline level of FSH
(follicular stimulating hormone)

Embryo Quality

Women with poor prognosis embryos, including conditions such as a thick zona pellucida, slow cell division rate, or high cell fragmentation

IVF Attempts

Women who have failed 1 or more IVF cycles

Frozen Embryos

Women using frozen/thawed embryos, which may have hardened zona pellucida