# DuraSlide™ Reusable Chambers

Hamilton Thorne is introducing the DuraSlide™ family of reusable sperm counting chambers for both CASA and manual counting.

## DuraSlide CASA

Featuring a depth of 20 µm and a non-gridded counting surface, the DuraSlide CASA is the perfect companion for the IVOS and CEROS sperm analysis systems. The kit includes 2 reusable coverslips.

## DuraSlide G10 & DuraSlide G20

For manual counting of sperm cells, the DuraSlide G10 and G20 feature an engraved counting grid with a depth of 10 µm and 20 µm respectively.

Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below). The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. Count all cells within this center square millimeter (1mm x 1mm area)

- For the DuraSlide G20, the ruled surface is 0.02 mm below the cover glass, so that the volume over a square millimeter is 0.02 cubic mm.
- For the DuraSlide G10, the ruled surface is 0.01 mm below the cover glass, so that the volume over a square millimeter is 0.01 cubic mm.