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Oosight Imaging System^®

The Oosight Imaging System provides high-contrast live images of the oocyte and spindle to determine which subpopulations are at high risk for producing chromosomally abnormal embryos and can prevent potentially damaging effects from injecting immature oocytes.

Our unique and patented solid-state, liquid crystal technology is an easy add-on to your ICSI workstation. Oosight software runs on your computer to capture, display, and analyze your images.

Snap and click to report data – it’s really that simple!

Oosight Imaging System is for research purposes only in certain territories.

Measures molecular order in either fresh or previously frozen oocytes to help with selection prior to ICSI or implantation
Safe, non-invasive imaging without the use of labels or stains
Seamless integration with HT laser systems

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Applications

Oocyte grading
SCNT (Somatic Cell Nuclear Transfer)
Stem Cell
Developmental Biology
Animal ART and Human Research

Specifications

Components	Oosight CCD Camera, LC Compensator Optic, Circular Polarizer/Interference Filter, C-Mount Adapter, Power Supply
Wavelength of Operation	546nm
Spatial Resolution	Diffraction limited
C-Mount	Demagnification C-mount within the range of 0.6x – 0.7x
Power Source	5V 3A with universal input voltage adapters
Image Output Format	TIFF
CCD Camera	Sensor Size 1/2-inch optical format Image Size 1024 x 1392 pixels Pixel Dimensions 4.65 x 4.65 μm Digital Output 8-bit Binning Modes 1×1, 2×2
Microscope Compatibility	Oosight systems are compatible with many research-grade microscopes, including those made with Leica®, Nikon®, Olympus®, and Zeiss®. Contact us for more information!
Laser Compatibility	Hamilton Thorne: XYRCOS, XYClone, LYKOS, ZILOS-tk
Environmental Requirements	Operating requirements: Indoor Operating temperature: 15 ˚C to 40 ˚C Operating humidity: 65%, non-condensing Operating altitude: 2000 meters Storage temperature: -10 ˚C to 55 ˚C Storage humidity: 100%, non-condensing Pollution degree: 2

Computer Requirements	Recommended	Minimum
PC Desktop/Laptop	i5, i7	Intel Pentium, 2 GHz
Operating System	Windows 10, 32- or 64-bit	Windows 7, 32-bit
Memory	4+ GB	1 GB
Hard Disk	250+ GB	80 GB
Display	1920×1080, 1920×1200	1280 x 1024
USB Ports	USB 2.0 2 available ports	USB 2.0, 2 available ports

Features & Benefits

Understanding the oocyte is critical to understanding embryogenesis, and studies show that an oocyte’s disrupted spindle apparatus or a weakened zona pellucida can yield lower pregnancy rates. In fact, studies show that pregnancy is up to 8 times more likely when the inner zona pellucida is well-ordered.¹

Unprecedented Resolution
High-contrast live images of the oocyte and spindle

Non-invasive Imaging
Does not require the use of any labels or stains

Quantitative Analysis
Ability to track oocyte behavior over time and record data points of molecular density and orientation

Proven
Successfully used to image many different mammalian species for both enucleation and developmental studies

Reporting Capability
Collected sample data can be organized into an intuitive, exportable report

Easy Integration with Small Footprint
Fits most inverted microscopes with simple installation process

Adding Oosight to your lab can improve oocyte quality grading success by providing a quantitative and reproducible method to measure biological disruption within the oocyte. The Oosight provides measurements of molecular order and alignment of birefringent structures including: the spindle, zona pellucida, and oolemma.

Improve the efficiency of cryopreservation

The Oosight helps verify vital structural bodies in the oocytes are intact after undergoing freezing procedures.

Nuclear Transfer (SCNT)- No Staining Needed

With Oosight, you can remove the spindle in nuclear transfer techniques without using Hoechst dye DNA staining and minimize damage to vital cytoplasmic organelles.

1. Shen Y, et. al. High magnitude of light retardation by the zona pellucida is associated with conception cycles. Human Reproduction, 2005 Jun; 20(6):1596-606

Software

Oosight can help identify oocytes that may have been falsely labeled MII due to conventional imaging techniques. The system can also help screen for oocytes with highly disrupted spindles, such as those that are multi-polar.

In a conventional contrast image (left) of a human MII oocyte taken just prior to ICSI, structures such as the spindle and multiple layers of the zona pellucida remain invisible. In an Oosight image (right) the spindle is clearly seen to be nicely barrel shaped and the three layers of the zona pellucida are all visible.

Oosight software visualization tools include (left to right): slow-axis orientation color map, orientation vector overlay, retardance color map, automated SpindleFinder™, and automated ZonaFinder™.

With unprecedented resolution and calibrated setup, Oosight software helps improve your grading routine with reproducibility and the speed needed for micromanipulation.

Choose between the Oosight Basic vs Oosight Meta system.

Oosight Basic

The Oosight Basic imaging system combines patented optical, electronic and software elements to produce both live and still images of critical structures within the oocyte, including the meiotic spindle and tri-laminar zona pellucida.

Oosight Meta

The Oosight Meta imaging system combines the image capture capability with additional analysis tools such as automated SpindleFinder™, automated ZonaFinder™, reporting, and Movie Capture to make quantitative, reproducible oocyte analysis possible.