GM508 CultActive

Specifications

• Bicarbonate buffered
• Ready to use
• For investigation of fertilization failure (deficient oocyte activation)
• Contains Ca2+-Ionophore A23187

Composition

• NaCl, KCl, KH₂PO₄, MgSO₄ CaCl₂
• Bicarbonate, EDTA
• Glucose, Lactate, Pyruvate
• Non-essential and essential Amino Acids, Alanyl-Glutamine
• Ca₂+-Ionophore A23187, DMSO

Instructions for Use

Prior to use, both GM508 CultActive and culture media (i.e.,GM501 Cult) must be equilibrated for 4 hours in vial not firmly closed at 5 – 7% CO₂ and 37°C prior to use.

Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use.

Recommended Application

• Prior to use, both GM508 CultActive and culture medium (i.e. GM501 Cult) must be equilibrated for 4 hours at 5-7% CO₂  and 37°C.
• Prior to equilibrating, remove vial stopper and loosely replace to allow vial to vent.
• Shake GM508 CultActive for approximately 30 seconds immediately prior to use.
• For each oocyte, prepare 1 drop (30 μl) GM508 CultActive (1# in diagram below) and 2 drops  (30-50 μl) MOPS-and HEPES-free culture medium (#2 and #3 in diagram below). An oil overlay is not necessary due to the short exposure time.
• Immediately after the ICSI procedure, incubate the oocytes for 15 minutes in the equilibrated CA²⁺-Ionophore GM508 CultActive drops (#1).
• Remove the oocytes from the GM508 CultActive drop and wash them twice in a HEPES-or MOPS-free culture medium (#2 and #3).
• Place the oocytes in culture media for further culture.
• Assess the development at desired time points. 
• Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use. 

Resources

Part Number

4 GM 508CULT-active1

Size

1 x 1 ml

Storage

2 – 8°C

Shelf Life

6 months

*From the date of manufacture