GM501 Wash

Indications for Use

Indications include oocyte and embryo washing (e.g. after oocyte aspiration, after hyaluronidase treatment to remove cumulus cells, before and after cryopreservation, and before embryo transfer) and micromanipulation procedures (e.g. assisted hatching). GM501 Wash is not intended for use in intrauterine insemination procedures or transferring embryos into the uterine cavity.

• The product can be used safely for up to 7 days after opening when stored at 2 – 8°C and sterile conditions are maintained.
• Includes highest available purity (Ph. Eu./USP) Human Serum Albumin (5.00 g/liter)
• Available in 20ml, 50ml and 500ml sizes

 Instructions for Use

• The medium must be equilibrated overnight in a humidified CO2 incubator (at 5-7%CO2, 37°C).
• For oocyte or embryo washing, prepare suitable culture dishes (MEA-tested) containing 15-100 µl drops under oil suitable for assisted reproductive techniques or larger volumes (0.5-1 ml) in appropriate dishes of GM501 Wash according to laboratory standards. Equilibrate the dishes overnight in a humidified CO2-incubator (at 5-7% CO2, 37°C) to ensure temperature and gas equilibration. For washing, pass the oocytes or embryos through several droplets of GM501 Wash according to laboratory practice. For micromanipulation procedures, apply one or two drops per oocyte or embryo, per laboratory practice.
• Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for media preparation and use.

Composition

• NaCl, KCl, KH₂PO₄, MgSO₄7H₂0, CaCl₂H₂0
• HEPES, EDTA
• D(+)-Glucose Anhydrous, Na-Lactate (50% solution), Na-Pyruvate
• Non-essential and essential Amino Acids, Alanyl-Glutamine
• Human Serum Albumin

Specifications

Order Codes

Resources