TOX IVOS^® II

Applications

• DART Studies  
• Drug Safety Assessments
• Preclinical studies 
• IND Enabling Studies 
• CROs (Contract Research Organizations) 
• Environmental toxicity studies 

Features & Benefits

TOX IVOS II (Integrated Visual Optics System) contains hardware and software that offers high-end performance while maintaining its ease of use. Spend less time on learning a new system with the IVOS® II’s intuitive Windows-based software platform. The settings for the integrated optics are made through the software and mechanical adjustments are rarely required.  

The fast automated stage provides precise temperature control and easy programmed sample positioning, perfect for repeated use with multiple technicians. Set the stage temperature from ambient to 40°C as the system remains constant to within the  0.5°C range. Program the stage for either manual or automatic field selection to fit your analysis workflow and operation.

Specifications

Color-coded Parameters and Interactive Illumination Check 

• View samples in high resolution with upgraded digital camera  
• Motile sperm identified by color-coded tracks and static sperm identified by red dot. 
• Unique color-coded Illumination helps optimize identification of the sperm head and tail.  
• Color-overlays on the playback image and the results may be turned on or off. 
• Auto Illumination available for use with rat sperm 

Analysis Results Updated in Real Time

• Data in the Results panel reflect the entire population of cells analyzed. Analysis results are updated in real-time as each field is added to the analysis. 
• Results are saved within image and video gallery. Re-analyze using saved settings or new set of parameters.  
• Selecting a track from the playback image opens up a zoomed image of the cell. Kinematic Measurements are relative only to the selected track. The video may be played or scrolled through frame by frame. 

Analysis Output

Simplify your research with customized parameters to meet your animal research needs! The intuitive HT CASA software provides quantitative results for the following parameters: 

• Total, Static, Motile, Progressive, Choice of billion or million 
• Concentrations: Total, Static, Motile, Progressive, (Choice of billons/ml or millions/ml) 
• Percentages: %Total, %Static, %Motile, %Progressive, %Slow 
• Kinematic Measures: DAP, DSL, DCL, VAP, VSL, VCL, ALH, STR, LIN, BCF, WOB (Average, SD, Median)  

Customized Reports

• Analysis results can be exported via ASCII file output.  
• Display sperm analysis results with customer user-friendly drag and drop report designer with unlimited number of templates.

21 CFR PART 11 Management Capabilities

Advanced User Management and Administrator Access  

• Advanced user creation functionality with security features preventing unauthorized users from accessing the software  
• Enhanced security with the password policy and password masking. 

Audit Trail and Secured Activity Log

• Audit Trail allows users to view and review the Audit Trail related to application functions and Part 11 Management, such as user management, group allocation, application functionality tracking, and parameter changes. with reasons for change noted.
• Administrative users can view all tracked activities and changes performed within the software. 

IDENT®

The IDENT® fluorescence module allows for total sperm counts to be made on the testicle tissue of interest in reproductive toxicology studies. By using the complementary IDENT® Stain – a specialized, DNA-specific, fluorescent dye, the system distinguishes all sperm within the homogenized tissue sample and calculates total numbers of sperm produced.

The analysis is easily visible under fluorescent lighting. This feature is included with every TOX IVOS system and can also be utilized for increased precision and accuracy of sperm detection again other debris, from media or semen sample sources.

• Interactive illumination saves time identifying sperm heads. Select the IDENT® Fluorescence setup and set the Minimum Brightness so that sperm heads appear blue on the screen.  
• The IVOS’s strobed illumination dramatically reduces phototoxic effects on the IDENT stained sperm by limiting exposure to UV light. 
• Fluorescent strobe minimizes bleaching of the stain and makes it possible to analyze fluorescently-stained, motile sperm (ideal for post-thaw motility studies).