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TOX IVOS^® II

The TOX IVOS II is a highly accurate, automated sperm analyzer that is an industry leader for Computer Assisted Sperm Analysis (CASA). Perform semen quality assessment for sperm count and concentration, as well as motility including classification of velocities, progressive and static and all kinematic measurements of motion.

For over 30 years, Hamilton Thorne has been helping researchers in pharmaceutical companies assess harmful effects in multi-species developmental and reproductive toxicology (DART) studies. Our TOX IVOS® II CASA system provides precise, objective, and repeatable results that you can trust.

Compatible with sperm samples of rat and all other species models for drug testing requirements
Live verification of sperm heads and tails with enhanced color-coded illumination
Optics combined into one integrated unit with heated stage and microscope
21 CFR Part 11 Management Capabilities with advanced user management and audit trail
High resolution digital imaging and video gallery of analysis fields

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Overview
Software
Tech Support & Service Plans
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Applications

DART Studies
Drug Safety Assessments
Preclinical studies
IND Enabling Studies
CROs (Contract Research Organizations)
Environmental toxicity studies

Features & Benefits

TOX IVOS II (Integrated Visual Optics System) contains hardware and software that offers high-end performance while maintaining its ease of use. Spend less time on learning a new system with the IVOS® II’s intuitive Windows-based software platform. The settings for the integrated optics are made through the software and mechanical adjustments are rarely required.

The fast automated stage provides precise temperature control and easy programmed sample positioning, perfect for repeated use with multiple technicians. Set the stage temperature from ambient to 40°C as the system remains constant to within the 0.5°C range. Program the stage for either manual or automatic field selection to fit your analysis workflow and operation.

Specifications

IVOS II	Dimensions: H: 11.1in (282mm) W: 20.3in (515mm) D: 19.3 (490mm) Weight: 53lb (24.1kg)
Objectives	Standard: 10x, 4x, 10x UV Optional: 20x, 40x, 60x, 100x
Image Type	Standard: Negative Phase Contrast, Darkfield Optional: Brightfield
Temperature Control	Heat from 20°C (Room Temperature + ~3°C) to 45°C
Compatible Specimen Chambers	Type: Slide, Cannula, Makler Depth: User defined
Objectives	Image Type: Dark field, Bright field, Phase Contrast
Image Acquisition	Frame Rate: 60 Hz Frames: 1 – 60 Field Selection: Automatic or manual
Power Requirements	Input voltage: 110-240 VAC Power: 350 watt (max) Line Frequency: 50/60 Hz

The TOX IVOS® II sperm analyzer is powered by our user-friendly HT CASA software with customized components to meet the strict guidelines required for the reproductive toxicology testing programs. Analyze sperm counts, concentrations, motile, and all categories of motion including progressive motility and kinematic measurements using set parameters or customized algorithms tailored to meet your research needs!

Complete your testing studies on both swim out or ejaculated samples, as well as homogenized tissue samples for total sperm production counts by tissue gram.

Color-coded Parameters and Interactive Illumination Check

View samples in high resolution with upgraded digital camera
Motile sperm identified by color-coded tracks and static sperm identified by red dot.
Unique color-coded Illumination helps optimize identification of the sperm head and tail.
Color-overlays on the playback image and the results may be turned on or off.
Auto Illumination available for use with rat sperm

Analysis Results Updated in Real Time

Data in the Results panel reflect the entire population of cells analyzed. Analysis results are updated in real-time as each field is added to the analysis.
Results are saved within image and video gallery. Re-analyze using saved settings or new set of parameters.
Selecting a track from the playback image opens up a zoomed image of the cell. Kinematic Measurements are relative only to the selected track. The video may be played or scrolled through frame by frame.

Analysis Output

Simplify your research with customized parameters to meet your animal research needs! The intuitive HT CASA software provides quantitative results for the following parameters:

Total, Static, Motile, Progressive, Choice of billion or million
Concentrations: Total, Static, Motile, Progressive, (Choice of billons/ml or millions/ml)
Percentages: %Total, %Static, %Motile, %Progressive, %Slow
Kinematic Measures: DAP, DSL, DCL, VAP, VSL, VCL, ALH, STR, LIN, BCF, WOB (Average, SD, Median)

Customized Reports

Analysis results can be exported via ASCII file output.
Display sperm analysis results with customer user-friendly drag and drop report designer with unlimited number of templates.

21 CFR PART 11 Management Capabilities

Advanced User Management and Administrator Access

Advanced user creation functionality with security features preventing unauthorized users from accessing the software
Enhanced security with the password policy and password masking.

Audit Trail and Secured Activity Log

Audit Trail allows users to view and review the Audit Trail related to application functions and Part 11 Management, such as user management, group allocation, application functionality tracking, and parameter changes. with reasons for change noted.
Administrative users can view all tracked activities and changes performed within the software.

IDENT®

The IDENT® fluorescence module allows for total sperm counts to be made on the testicle tissue of interest in reproductive toxicology studies. By using the complementary IDENT® Stain – a specialized, DNA-specific, fluorescent dye, the system distinguishes all sperm within the homogenized tissue sample and calculates total numbers of sperm produced.

The analysis is easily visible under fluorescent lighting. This feature is included with every TOX IVOS system and can also be utilized for increased precision and accuracy of sperm detection again other debris, from media or semen sample sources.

Interactive illumination saves time identifying sperm heads. Select the IDENT® Fluorescence setup and set the Minimum Brightness so that sperm heads appear blue on the screen.
The IVOS’s strobed illumination dramatically reduces phototoxic effects on the IDENT stained sperm by limiting exposure to UV light.
Fluorescent strobe minimizes bleaching of the stain and makes it possible to analyze fluorescently-stained, motile sperm (ideal for post-thaw motility studies).